PrintedPages:3 PaperID: 15401 Roll Number EI\D SEMESTER EXAMINATION, 201+15 B.TECH.(SEMESTER_13) BTY 311: ANIMAL BIOTECIILOLOGY Time:03Hours Mar...
7 downloads
18 Views
204KB Size
PrintedPages:3 PaperID: 15401
Roll Number
EI\D SEMESTER EXAMINATION, 201+15 B.TECH.(SEMESTER_13) BTY 311 : ANIMAL BIOTECIILOLOGY
Time:03Hours
Mar
Note : 1. Attempt all sections. 2. All questionscarryequal marks.
NOTE: ATTEMPTANY TWO QUESTIONS. Q.l Answer all parts of the following:
(5x 4 = 20)
(i)
Discuss the methodsused to sterilizethe equipmentsand the componentsof culture medium.
(ir)
Discuss briefly the natr.ual,definedandartificial media.
(iii) Differentiate betweencontinuouscell cultureand finite culture. (iv) What do you urderstand by gene transfer? Mention the applicationsof geneftansfer. (v)
Enlist ethical issuesin cell culture.
,l
I i
[C ontd.......
Ansr.vcr:rll parts ol'the following:
(5x4 = 20)
(i)
[)iscussbriefly the varioustechniquesused in freezestorageand the transportationof aninal cell culture.
(ii)
Ilxplain Organotypic and histotypic ell culture in brief.
(iii)
What are the advantagesof bone maffow culture?
(iv)
Discuss briefly the steps involved in propagation of stem cell lines with neat sketch
(")
Give a brief account on advantages and disadvantages of glycerol based freeze storage compared to the DMSO based storageof the cell lines.
Answer all parts of the following:
(ii)
write down the advantages of anchorageindependent celtrsover that of anchoragedependentcells" Discuss with a suitable examplehow all the anchorageindependentcells behavein a remarkablysimilar mannerirrespectiveof their origin?
Q.5 Answerboth parts of the following: (i)
Discuss the diffrculties and strategiesfor developingthe cell iines of animal tissues.
(ii)
Describethe selectioncriteria for choosing a.suitablemedium for animal cell culture in the laboratory.Describesomedefined mediawith and without serum?
Q.6 Answer both parts ofthe following: (5x4=20)
(i)
Write the composition of RMPI and Eagle's MEM.
(ii)
Discuss various applications of stem cell lines.
(iii)
Suggest few growth regulators that minimize the problem of contact inhibition in cells.
(iv)
Write about Cloning of endangeredanimals.
(")
Write a brief note on viral sensitivitv of cell lines.
(2xt} = Z0)
Qxl0 =20)
(i)
Describe briefly the scope of animal cell and tissue curture. Discussthe major advantages and disadvantages of animartissue culture in detail.
(iD
what do you understandby the stem cell lines?Discussbriefly the steps involved in propagationof stem cell lines with neat sketch.
Q.7 Discuss the basic concept of cloning of cell lines. Comparecloning from embryonic cells to that from adult cells. Discussrelative merits TE: ATTEMPTANY TWO QUESTIONS. Answer both parts of the following: (i)
of eachof theseapproaches. (2x10=20)
Discuss briefly how this technology has raised a hope to provide tailor made solution for curing a disease to an individual.
IContd
(20)
