PaperID: 3475 Time : 03 Hours Roll Number PrintedPages: 3 MaximumMarks: 100 END TERM EXAMINATION, 2012-t3 B.TECH.(TERM-o4) BTY 205: ANIMAL BIOTECHNOLO...
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END TERM EXAMINATION, 2012-t3 B. TECH. (TERM-o4) BTY 205 : ANIMAL BIOTECHNOLOGY Time : 03 Hours
Maximum Marks: 100
Note : 1. Attempt all sections. 2. All questionscany equal marks.
NOTE: ATTEMPTANY TWO QUESTIONS. Q.l Answer all parts of the following: (i) Describethe processof "transfection"' (ii)
(5x4=20)
cell culture' Explainthe primaryand established
(iiD What is haematoPoiesis? (iv) Explain in vitro clonal assay' (v)
DefinePleuriPotencY.
[C ontd......
Q.2 Answer all parts of the fbllowing: (i)
What are the objectives of aseptictechniques?
(ii)
Define histotypicculture.
(iii)
What is transgenesis?
(i")
Define the types of ceil cloning.
(v)
Ilxplain suspensionculture.
Q.3 Answer all parts of the following:
(5x4 = 20)
Q.s Answer both parts of the following: (i)
Explain the differentiation potential of stenr cells. Discuss applicationsof stemcell technology.
(ii)
Enumerate the various bio-ethical issues in application of animal biotechnology.
Q.6 Answer both parts of the following: (5x4=20)
(i)
Explain the processof trypsinization for disaggregation.
(ii)
Explain how somatic all nucleadtransferis done.
(iii)
Enumeratethe application of "hybridoma technology'.
(i")
Explain the various physical and chemical factors required for animal cell cuiture.
(v)
Explain engineered embrayonic stem cell method is develop transgenicmice.
(2x10= 2O)
(2x10=20)
(i)
Explain the processof "embryonic stemcell culture".
(ii)
Discussin detail "the maintenanceof cell culture".
Q.7 Explain the processin the developmentof transgeniccattle (sheep/ goat/cow). (20)
NOTE: ATTEMPTANY TWO QUESTIONS. Q.4 Answer both parts of the following:
(2x10=20)
(D
Write in detail the "applications of animal cell".
(ii)
Enumerate the role of serum and supplements in animal cell culture.
[Contd.......
