Basic plant biotechnology. (BTY 309).B.Tech.Sem-13, 2014-15

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EI\D SEMESTER EXAMINATION, 2014-15 B.TECH. (SEMESTER-13) BTY 309 : BASIC PLANT BIOTECHNOLOGY

Time : 03 Hor.rs

MaximumMarks : 100

2. AII questions carryequalmarks.

NOTE: ATTEMPTAIiY TWO QUESTIONS. Q.l

Answer all parts of the following:

(5x4=20)

(i)

Discussthe componentsof basicplant tissueculturemedia.

(ii)

Write a note on Micropropagation.

(iii)

write the steps of sterilization of shoot tip explant and explain the role of Bavistin being usedthere.

(lD

Write the differencesbetweenshootbud and somaticembryo?

(v)

What are somaclonalvariants?Mention their applications.

[C ontd.......

Arrswerall parts of the following: (i)

(ii)

(5x 4 = Z0)

What are synthetic and natural Cytokinins? Mention their names & fur-rctions. Explain the techniquesof viability testing for cells in suspension cultures.

(iii)

What is cellular totipotency? What is de-differentiation?

(i")

What

are

the

differences

between

embryogenesis

and

organogenesis? (")

(5x4=20)

(i)

Differentiatebetweensomaticembyosandzygoticembryos.

(ii)

what are artificial seeds?Mention the steps taken for their production

(iii)

write briefly the role of micronutrientsbeing usedin planttissue culturemedia.

(i")

What is in situ andex silz conservation? Explain.

(")

How doessomatichybrid differ from cybrid?

fE: ATTEMPTANY TWO QUESTIONS. Answerboth parts of the following:

(2x10=20)

Discussthe advantages and limitationsof plant tissueculture. IContd

Describethe methodsof protoplastisolationandpurification?

Q.5 Answerboth parts of the following:

(2x 10= 20)

(i)

What is somatichybridization? Whatis its utility? Explain"

(ii)

Discuss

applicability of Bioreactors in plant cultivation through tissueculture.

Q.6 Answerbothpartsofthefollowing:

(2x10=20)

(i)

what is cybridization?what are the variousmethodsto obtain cybrids?

(ii)

DescribeBergmann'scell plating techniqueand paperraft nurse techniqueincluding diagrams.

What is cytodifferentiation? Explain.

Answer all parts of the following:

(i)

(ii)

Q.7 what is androgenesis? Discuss direct and indirect androgenesis? Discussthe methodsandapplicationsof haproidproduction. (20)