(ST-2_05_EN) Modern Analytical Techniques

Modern Analytical Techniques

ST-2/05_EN Year of study

I

semester

Number of teaching hours (15 weeks)

W lectures

2

45

1

Lecturer:

Course type (number of hours per week) Ć exercises

S seminar

L laboratory

2

P project

Assessment method

ECTS credits

Z

4

dr hab. inż. Adam Grochowalski, prof. PK

Programme description: Description of common terms in instrumental analysis: precision, accuracy, selectivity, sensitivity, traceability, detection limits, signal to noise. Electroanalytical Methods: Brief review of electrochemistry. Standard electrode potentials. Potentiometric methods . Reference electrodes. Ion selective electrodes. Membrane electrodes. Sensors. Voltammetry and Polarography. Dropping Mercury electrodes. Tast Polarography. Pulse Polarography. Spectroscopic Methods: Review of properties of electromagnetic radiation. Atomic Spectroscopy, Beer's Law. Instrumental components (detectors, monochromators). Double beam spectroscopy. Diode array detectors. Atomic absorptive and emission spectrometry. Comparison of Atomic Absorption and Flame Emission techniques. Inductively coupled plasmas (ICP). Chromatographic analysis: The principles of chromatography. Types of chromatography used in modern laboratory. Partition coefficients, Plate theory - optimization of performance. Van Deemter equation Retention times. Gas Chromatography: Columns and stationary phases, detectors, examples of use. Liquid Chromatography: HPLC Principles and applications. Chiral columns. Ion-chromatography Capillary Electrophoresis. Mass spectrometric detectors in trace organic analysis (high resolution MS, MS/MS, TOFMALDI, ITD etc.). Hyphenated techniques like LC-MS, GC-MS, HPLC-FTIR. Gel Permeation and Filtration. Sample preparation for chromatographic analysis. Result calculation and statistical evaluation.

Training effect – skills and competence: Understanding of current concepts in analytical methodology, equipment, and procedure, both in their utility and their limitations. Hands-on experience in analytical techniques that can be transferred to other disciplines in science. Experience in analyzing experimental results at various levels of sophistication, ranging from purely qualitative to highly quantitative. Skills in quality assurance quality control (QA/QC) in instrumental analytical chemistry.

The lecturer informs about requirements, weight of assessment components, recommended literature, schedule of tests or other organizational issue at the first meeting.