The lecturer informs about requirements, weight of assessment components, recommended literature, schedule of tests or other organizational issue at t...
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ST-2/12l_EN
Preparative and Analytical Liquid chromatography
Year of study
semester
Number of teaching hours (15 weeks)
I
2
45
Lecturer:
Course type (number of hours per week) W lectures
Ć exercises
S seminar
L laboratory
1
2
P project
Assessment method
ECTS credits
Z
3
dr inż. Sławomir Wybraniec
Programme description: Basic chromatographic parameters. Normal and reversed phase systems. Kinds of chromatographic techniques. Isocratic and gradient elution. Eluent selection. Types of analytical and preparative columns. Preparative column techniques. Types of chromatographic sorbents. Setting of optimal preparative separation conditions. Preparative separation of natural compounds. Detection in liquid chromatography. Modern liquid chromatographic devices.
Training effect – skills and competence: Operation on modern devices of liquid chromatography. Preparation of samples for chromatographic analyses and for preparative isolation of compounds. Selection of eluent composition and planning eluent gradient. Selection of basic parameters for chromatographic separation and compound detection. Prediction of influence of chromatographic parameters on separation of compounds. Efficient monitoring of composition of obtained preparative fractions. Preparative isolation of natural compounds from complex matrices. Learning of techniques of eluate concentration. Computer assisted elaboration of obtained chromatograms in simple and complex chromatographic programs.
The lecturer informs about requirements, weight of assessment components, recommended literature, schedule of tests or other organizational issue at the first meeting.
