Recombinant DNA technology. (BTY 310).B.Tech.Sem-13, 2014-15

Printed Pages : 4

Paper ID: 15400

Q.7 How would you prepaxe a cDNA library from rat liver? What strategywould you apply to screenthe library to get a gene of your interest? Q0)

RoIl Number

END SEMESTER EXAMINATION, 2014-15 B.TECH. (SEMESTER-13) BTY 310 : RECOMBINANT DNA TECHLOLOGY Time : 03 Hours Note:

MaximumMarks : 100

1. Attemptall sections. 2. AII questionscarry equal marks. I i i + r t r ++ s

. .

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S'ECTION-A NOTE: ATTEMPTANY TWO QUESTIONS. (5x4=20) Q.l Answer all parts of the following: (i) what different information does Gel DocumentationSvstem provides?

(ii)

What arecriteria for RestrictionEndonuclease nomencla

?

(iii) what is the principle of silica column-basedDNA isolation technique? (iv) Draw and label the different parts of a prokaryoticC-Terminal ExpressionVector. (v)

4

What is GeneTherapyand how the techniqueis presentlyused with SuicideGenes? 1

IContd

Q.2 Answer all parts of the followins: (1)

(5x4=20)

what are high-fidelity themostable DNA polymerases? Name two such DNA porymerasesalong with names of their source orgalllSms.

(ii)

what are the differencesbetweenprokaryotic and yeast-based expression vectors?

(iii) How PCR techniqueis successfully used in variousmedicaland agricultural diagnostic kits? (iv)

Why

agarose

blotting

gels

techniques

NOTE: ATTEMPTANYTWO QUESTIONS. Q.4 Answerboth parts of the following: (i)

pretreated only for Southern and not for Northern blotting

are

Why Nylon membranes having only positive charge are used to transfer nucleic acids from gels?

).3 Answer all parfs of the followins: (i)

(ii)

Why

denatured

agarose gels

(ii)

Q.5 Answer both parts of the following:

(2x10= 20)

(D

Mention the different DNA rabelingtechniques.Describe any one of them with suitablediagrams.

(ii)

How the discoveryof ribozymeshelpedin developingthe RNAWorld hypothesis?

(5x 4 = 20) are

used

only electrophoresisand not for DNA electrophoresis?

for

RNA

what is IMAC? How the techniqueis used to purifu histidinetaggedrecombinantproteins?

(iv) what are the differences among plasmids, phagemids, fosmids andcosmids? Describe the structure and mode of polymerases. Z

what is TA cloning and how is it performed?Showwith the help of a diagram.

Q.6 Answer both parts of the following:

action of

Qx10=20\

(i)

what is Next GenerationDNA Sequencing? Mention any one of the Next Generation Sequencing techniques with suitable diagrams"

(iD

what is alpha-complementation? How it is used to determine positive clones in transformation experiments? show with suitablediagrams.

(iii) what are the distinctive properties of prasmids as croning vectors?

(")

What is Restriction-Modification System? Mentionthe different types of R-M systems?

techniques? (v)

(2x10=20)

DNA

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